PNC-27

Chemical Identifiers & Names

• Name: PNC‑27
• CAS Number: 1159861‑00‑3
• Molecular Formula: C₁₈₈H₂₉₃N₅₃O₄₄S
• Molecular Weight: ~4,031.7 Da

Amino Acid Sequence

H‑Pro‑Pro‑Leu‑Ser‑Gln‑Glu‑Thr‑Phe‑Ser‑Asp‑Leu‑Trp‑Lys‑Leu‑Leu‑Lys‑Lys‑Trp‑Lys‑Met‑Arg‑Arg‑Asn‑Gln‑Phe‑Trp‑Val‑Lys‑Val‑Gln‑Arg‑Gly‑OH

(32 amino acids: p53(12–26) binding domain + cell-penetrating “MRP” sequence) :contentReference[oaicite:1]{index=1}

Synthesis & Purity

• Synthesized via solid-phase peptide synthesis
• Purity: > 95 % (HPLC and MS verified) :contentReference[oaicite:2]{index=2}

Structural Properties (NMR & 3D conformation)

• 32-residue peptide with three α-helical domains linked by flexible loops; forms “S-shaped” conformation in aqueous solution :contentReference[oaicite:3]{index=3}
• Adopts “U-shaped helix–coil–helix” in membrane-mimetic environments; amphipathic with hydrophobic and polar faces :contentReference[oaicite:4]{index=4}
• NMR structure superimposable on p53‑HDM2 binding motif; coordinates available as PDB entry 1YCR :contentReference[oaicite:5]{index=5}

Physicochemical Properties

• Approx. 32 amino acids; estimated rotatable bonds ~90+
• Complex amphipathic structure with alternating hydrophobic and charged residues :contentReference[oaicite:6]{index=6}
• Various fluorophore-labeled variants (e.g., FITC, TAMRA) synthesized with ≥95 % purity for structural studies :contentReference[oaicite:7]{index=7}

Additional Chemical Data

• Also available in trifluoroacetate (TFA)–free powder form; purity ~96 %, MW 4,031.7 Da :contentReference[oaicite:8]{index=8}

References

1. Sarafraz-Yazdi E, et al. “Anticancer peptide PNC-27 adopts an HDM‑2-binding conformation and kills cancer cells by binding to HDM‑2 in their membranes.” Proc Natl Acad Sci U S A. 2010;107(5):1918–1923. :contentReference[oaicite:9]{index=9}
2. Sarafraz‑Yazdi E, et al. “PNC‑27…structure, pore formation…” Biomedicines. 2022;10(5):945 (PDB 1YCR). :contentReference[oaicite:10]{index=10}
3. Do PM et al. “NMR solution structure of a peptide from the mdm‑2 binding domain of the p53 protein…” J Biol Chem. 2004;279(20):21218–21226. :contentReference[oaicite:11]{index=11}
4. PMCID: PMC2836618 – peptide synthesis details & purity via HPLC/MS. :contentReference[oaicite:12]{index=12}
5. Novopro Labs product documentation: sequence, formula, weight, purity. :contentReference[oaicite:13]{index=13}
6. ChemicalBook listing (CAS, sequence, formula). :contentReference[oaicite:14]{index=14}