FOXO4 10mg

FOXO4 (Forkhead Box O4)

• Gene symbol: FOXO4 (also AFX1, MLLT7); located on X chromosome
• Protein length: 505 amino acids
• UniProt ID: P98177
• Transcript (canonical): ENST00000374259 / NM_005938.4
• Domain architecture:
  • N‑terminal regulatory region (phosphorylation / 14‑3‑3 binding sites)
  • Forkhead (winged‑helix) DNA-binding domain (~110 aa)
  • Nuclear localization signal (adjacent to FHD)
  • C‑terminal transactivation domain (TAD)
• FHD structure: Helix‑H3 docks in DNA major groove; H2–H3 loop and water networks contribute to sequence specificity (1.9 Å crystal structure)
• NMR insights: CR3/TAD region dynamically interacts with FHD during DNA binding
• Regulatory modifications: Phosphorylation, acetylation, ubiquitination, 14‑3‑3 binding affect localization and activity

References (academic)

1. Boura E *et al.*, “Structure of the human FOXO4‑DBD‑DNA complex…”, *Acta Crystallogr D Biol Crystallogr.* 2010;66(Pt 12):1351‑7. DOI:10.1107/S0907444910042228.
2. Kang D *et al.*, “NMR investigation of FOXO4‑DNA interaction…”, *Commun Biol.* 2024;7:1425. DOI:10.1038/s42003‑024‑07133‑1.
3. Silhan J *et al.*, “14‑3‑3 Protein Masks the DNA Binding Interface of FOXO4”, *J Biol Chem.* 2009.
4. Obsil T & Obsilova V.*, “Structural basis for DNA recognition by FOXO proteins”, *BBA.* 2011;1813(11):1946‑53.
5. UniProt Consortium. *UniProt P98177 (FOXO4_HUMAN)*.
6. Fitzgerald SJ *et al.*, “Minireview: Mechano‑growth factor…”, *Endocrinology.* 2010;151(3):865‑875.